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KMID : 0359319910310030311
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Korean Journal of Veterinary Research
1991 Volume.31 No. 3 p.311 ~ p.318
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Studies on the cloning gp50 and gp63 genes of Pseudorabies virus ( Shope strain )
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송재영/Song, Jae Young
이영순/권창희/안수환/Maeda, Susumu/김병한/이중복/Lee, Yong Soon/Kweon, Chang Hee/An, Soo Hwan/Kim, Byoung Han/Lee, Jung Bok
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Abstract
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The DNA fragment representing for Pseudorabies gp50 and gp60(Shope) was cloned by recombinant techniques. The viral DNA was extracted from the infected cells and digested with Bam HI. The 6. 8 Kb of Bam HI fragment was isolated from agarose gel and furtlier digested with Nde I folloaved by Klenow treatment. The blunt ended 4.9Kb fragment was cloned into pTZ18R plasmid vector. The upstream region of gp50 was further manipulated to remove its 5´ promoter region and create EcoRl site for possible eukaryotic expression system. The result of partial sequencing of cloned DNA indicated that Shope strain showed 95% homology with gp50 of Rice strain.
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KEYWORD
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